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KMID : 0903519720150030199
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
1972 Volume.15 No. 3 p.199 ~ p.206
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A Study on the Konjak Mannan - hydrolyzing Enzymes from Aspergillus awamori
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Abstract
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As a study on the konjak mannan-hydrolyzing enzymes from Aspergillus awamori, the culture conditions for enzyme formation, purification and properties of the enzymes and the effect of gamma-irradiation on the enzymatic hydrolysis were investigated. The results are summarized as follows:
1) A strain of A. awamori was selected as having the highest productivity of mannanase among 81 species of molds.
2) The optimum conditions for solid culture on wheat bran were 3 days of culture period, pH 4 of spraying water and 100% addition of tap water.
3) The optimum conditions for shaking culture were 6 days of culture period, addition of 0.1% xylose plus 0.5% konjak mannan and of 0.04% peptone.
4) Konjak mannan-hydrolyzing enzymes were separated into fraction I and fraction II by ammonium sulfate fractionation and DEAE-Sephadex column chromatography.
5) Fractions I and II showed pH optima of 4, pH stability of 3.5∼5 and 3∼6 and the extent of hydrolyzing konjak mannan 9% and 50%, respectively.
6) Hydrolysis of konjak mannan by a crude enzyme preparation was partially accerelated by gamma-irradiation of substrate above 0.5 Mrad and the effect was more remarkable by irradiating in wet state than in dry state.
7) Gamma-irradiation of konjak mannan brought about the increase in reducing power and decrease in viscosity and the effect was more remarkable in wet state than in dry state.
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